Cotinine (COT), is an alkaloid found in tobacco and is also a major metabolite of nicotine The word "Cotinine" is an anagram of "nicotine". Cotinine is used as a biomarker for exposure to tobacco smoke.
Tobacco smoking results in the absorption of nicotine through the lung and buccal/nasal epithelium, after which nicotine is metabolized into about 20 metabolites excreted in urine.
Cotinine, a major metabolite, accumulates in the body with regular smoking. It is reported that Cotinine is stable in body fluids and has a relatively long half-life of approximately 17 hours. Therefore, the detection of Cotinine is less dependent on the time of sampling than that of nicotine and other metabolites.
- Cotinine levels <10 ng/mL are considered to be consistent with no active smoking
- 0 ng/mL to 100 ng/mL are associated with light smoking or moderate
- Levels above 300 ng/mL are seen in heavy smokers - more than 20 cigarettes a day
Cotinine assays provide an objective quantitative measure that is more reliable than smoking histories or counting the number of cigarettes smoked per day.
Test Options for Nicotine Testing:
- Urine - A lateral flow, one-step immunoassay for the qualitative detection of cotinine
- Saliva - Qualitative detection of Cotinine in oral fluids. It also permits the measurement of exposure to second-hand smoke
Your choice of either a Nicotine urine test kit which uses a dropper to carry drops of the urine specimen to the device for testing and results. The other option available would be the iScreen OFD, Cotinine saliva test method which utilizes antibodies to selectively detect elevated levels of nicotine in oral fluid. The Nicotine urine test is much more affordable but at the same time, the saliva device does not require a bathroom and the procedure can be completed without any difficulty at a desk within an office setting. Both test kits are completely disposable and should be discarded after use. if necessary, make a photocopy displaying your test results.